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dc.creatorRobeson,James
dc.creatorSkarmeta,Ana María
dc.date2000-12-01
dc.date.accessioned2019-06-20T13:59:51Z
dc.date.available2019-06-20T13:59:51Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-19572000000100001
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/99357
dc.descriptionAn IncP plasmid probe (pUCV2), coding for Cm-r, Km-r and bearing Ap (am) and Tc (am) resistance determinants was constructed by transposition of Tn9 (Cm-r) onto plasmid pLM2 for an efficient selection of potential recipients among natural marine bacteria. Using a Dap- E. coli donor, transmission of pUCV2 to marine bacteria was tested. pUCV2 is transferred to about 4-8% of natural, marine bacterial cells capable of forming colonies on a low nutrient, marine agar medium. The following bacterial genera, commonly found in the marine environment, could be detected when twenty of the transconjugant colonies obtained were identified: Vibrio, Pseudomonas and Aeromonas.
dc.formattext/html
dc.languageen
dc.publisherUniversidad de Valparaíso. Facultad de Ciencias del Mar
dc.relation10.4067/S0718-19572000000100001
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceRevista de biología marina y oceanografía v.35 n.2 2000
dc.subjectHorizontal transfer
dc.subjectIncP probe
dc.subjectmarine bacteria
dc.titleProbing transfer of an IncP replicon to natural marine bacteria


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