The fish feed used in aquaculture systems has many nutritional factors like protein, fat, fiber, ash, and humidity; from these factors, one of great importance is protein. The protein of fish feed is obtained from fish meal, and the demand for fish meal has increased; this induces to increase the price. Many pieces of research have been done about this topic to mitigate this problem. This research shows the differences between three commercial foods of the different processes with the same quantity of protein, using tilapia Oreochromis niloticus, to prove any difference between NO2-, NO3-, and NH4+. Nitrogen compounds were determined with HACH methods for the DR6000 spectrophotometer. Biometric data were registered throughout the growth of fish, along with measure aerobic metabolism. Results have shown that has better outcomes related to low excretion of nitrogen compound was medium-cost fish feed (MCFF). It also has sound effects associated with the length-weight relationship; that could be connected to these fish feed's protein sources.