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dc.creatorAsencio-Alcudia,Gloria Gertrudys
dc.creatorTovar-Ramírez,Dariel
dc.creatorLatisnere-Barragán,Hever
dc.creatorMartínez-García,Rafael
dc.creatorContreras-Sánchez,Wilfrido Miguel
dc.creatorAlvarez-González,Carlos Alfonso
dc.date2018-12-01
dc.date.accessioned2019-05-03T13:28:25Z
dc.date.available2019-05-03T13:28:25Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000500909
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/88486
dc.descriptionABSTRACT: Mexican snook (Centropomus poeyi) is one of the most important commercial species in Southeast Mexico, which has been overexploited by fisheries. For this reason, some recent studies related to its culture have been done. However, the basic biological knowledge of the digestive physiology is still unknown. In this study, the gene expression of trypsin, (TRY), bile-salt dependent lipase (BAL), amylase (AMY), and cytosolic non-specific dipeptidase (CNP) was investigated during the larval period from embryo (0 days after hatching, DAH), eleuteroembryo (1 DAH) until 25 DAH using RTq-PCR approach and was normalized with the elongation factor 1-( gene (EF1-α). TRY gene expression showed the maximum peak at 17 DAH; meanwhile, BAL gene expression showed variations during larviculture, however, three peaks were observed at 10, 17 and 25 DAH, which appears to correspond to changes in live food supply (rotifers and Artemia nauplii). AMY gene expression was low detected from hatching onwards and showed a slight peak at 10 DAH when larvae were fed with Artemia nauplii. Finally, CNP gene expression had presented three peaks at 2 DAH (yolk-sac absorption), 19 and 23 DAH when larvae were fed with Artemia nauplii. We conclude that C. poeyi larvae showed early and high digestive enzyme expressions, especially TRY, which indicate that could be possible to start the weaning process at 25 DAH, which could be shorter compared than other Centropomid species.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
dc.relation10.3856/vol46-issue5-fulltext-5
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceLatin american journal of aquatic research v.46 n.5 2018
dc.subjectCentropomus poeyi
dc.subjectsnook
dc.subjectlarviculture
dc.subjectgene expression
dc.subjectRT-qPCR
dc.subjectdigestive enzyme
dc.titleGene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi


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