Teaching vocation is an important factor considered as a basic competence in most of the teacher training programs in Chile. here is little research addressing this issue, which led researchers to carry out this study through the construction of a questionnaire to measure teaching vocation in pedagogy students (TVQ-S). he questionnaire was built based on a comprehensive literature review and process of content analysis by referee criteria and levels of internal validation with an instrumental study. It was made up of 37 items distributed in 6 factors that measure the interest in teaching, academic achievement motivation, self-determination, intrinsic motivation, disciplinary interest and a scale of lies. he Pilot study involved 209 student teachers from the University of Bio-Bio, Chile, including 86% women and 14% men, aged between 19 and 29 years. Statistical analyzes indicate a good level of overall reliability (α = .833) as such as its factors (.419 and .799); factors also have a significant level of correlation with global instrument (between .539 and .760 Pearson, significant p> .001). It can be concluded that the instrument is valid and reliable for assessing the construct teaching vocation in Chilean students of pedagogy.KEYWORDS. Teaching vocation; pedagogy; psychometric properties; assessment.doi: 10.21703/rexe.20181734cossa61